TB-500 5MG

Description

TB-500 5mg research peptide — Canada

Researched & reviewed by the SPO Quality Team · Last reviewed: 30 September 2026

“TB-500” is a product name that vendors have applied to two related peptides: thymosin β4, the 43-residue actin-binding peptide found in most mammalian cells, and Ac-LKKTETQ, a seven-residue acetylated piece of it (residues 17–23) that carries the actin-binding motif^{[1, 3]}. The independent report for this 5mg batch assigns the material as TB-4, meaning the full-length peptide. It is one sealed vial of lyophilized powder, supplied for laboratory research only.

Full research overview, study table & references → TB-500 in Canada: research guide

Identity at a glance

Identity values from PubChem and ChEMBL, sequence position from UniProt P62328, read on 30 September 2026.

This batch: certificate of analysis

Product tested	TB-500 5 mg (this listing)
Testing laboratory	Janoshik Analytical (independent)
Report number	#219841
Sample received / analysis date	13 August 2026 / 20 August 2026
Result line	TB-500 (TB-4)
Measured content	4.52 mg per vial (declared 5 mg)
Purity	99.371%
Report	View the report image · COA library

The measured content sits below the 5 mg declaration; the report states the number, and we print it as it stands rather than the label value. Purity and identity are separate measurements — see our HPLC vs. mass-spec guide — and the batch number on your label should match the report before use.

What researchers have looked at

• Endothelial cells and chick aortic arches: the seven-residue actin-binding motif alone matched the whole peptide’s migration and sprouting activity at about 50 nM, and soluble actin blocked it^[1].
• Rat full-thickness wounds: topical or intraperitoneal thymosin β4 raised re-epithelialisation by 42% at day 4 and 61% at day 7 against saline, with more collagen and vessels^[2].
• Rat Achilles transection (2026, n=32): the TB-500 group had higher load to failure and better histology scores than controls at four weeks; adding BPC-157 gave no extra benefit^[4].
• Limits: six months of thymosin β4 in dystrophic mdx mice increased regenerating fibres but did not improve strength, heart function or fibrosis^[5]; the human data are small investigational trials of thymosin β4, including a phase I safety study in 84 volunteers, with no approved product anywhere^[6]. The fragment is treated as a suspected doping agent in the analytical literature^[3].

Storage and handling

• Store the sealed vial at −20 °C for the long term (the site’s guidance: up to 24 months as dry powder); cool, dry and dark for short periods. Avoid freeze–thaw cycling.
• Water-soluble. Published rat studies used saline as the vehicle^[4]; laboratories also reconstitute with sterile or bacteriostatic water. Solutions keep less well than powder. Protocols are the researcher’s own and are not published here.

Also listed: TB-500 10MG (no batch report posted yet) · BPC-157/TB-500 10MG. Ships from inside Canada by Canada Post; the shipping policy has dispatch cut-offs, delivery times and the free-shipping threshold. The full catalogue is on our buy peptides online in Canada page.

Frequently asked questions

Can I buy TB-500 5mg in Canada?

Yes. This 5mg vial ships from inside Canada by Canada Post, so there is no customs hold or duty. It is supplied for laboratory research only; neither thymosin β4 nor the TB-500 fragment has an approved product in Canada, the US or the EU.

Which molecule is in this vial: thymosin β4 or the fragment?

The batch report for this listing (Janoshik #219841) labels the result line “TB-500 (TB-4)”, i.e. the laboratory assigned it as thymosin β4. The 7-amino-acid fragment Ac-LKKTETQ (889 Da) that has been found in other TB-500 products (PMID 22962027) is a different molecule; check the mass-spectrometry line on your own batch’s report.

How was this TB-500 batch tested?

Independently, by Janoshik Analytical: HPLC for quantity and purity and mass spectrometry for identity. Report #219841 (analysis 20 August 2026) states 4.52 mg measured content against a 5 mg declaration and 99.371% purity. Match the batch number on your label to the report in the COA library before use.

How should TB-500 5mg be stored?

As sealed lyophilized powder at −20 °C for long-term storage (the site states up to 24 months), or cool, dry and dark for short periods. Once dissolved the peptide is less stable; published rat work used saline as the vehicle (PMID 42542926). No dosing or administration guidance is provided.

How does TB-500 compare with GHK-Cu, the copper peptide it is often paired with in tissue-research literature? See TB-500 vs GHK-Cu: an actin-binding fragment and a copper carrier.

Cited

1. Philp et al. (2003), FASEB journal : official publication of the Federation of American Societies for Experimental Biology — PMID 14500546
2. Malinda et al. (1999), The Journal of investigative dermatology — PMID 10469335
3. Esposito et al. (2012), Drug testing and analysis — PMID 22962027
4. Biçer et al. (2026), Joint diseases and related surgery — PMID 42542926
5. Spurney et al. (2010), PloS one — PMID 20126456
6. Wang et al. (2021), Journal of cellular and molecular medicine — PMID 34346165

For laboratory research use only. Not for human or animal consumption. No dosing, administration or protocol information is provided, and nothing here is a claim of efficacy or safety in humans.